Combined Approach for Characterization of Uncultivated Magnetotactic Bacteria from Various Aquatic Environments
نویسندگان
چکیده
منابع مشابه
Combined approach for characterization of uncultivated magnetotactic bacteria from various aquatic environments.
Both magnetic collection and "race track" purification techniques were highly effective for selective enrichment of magnetotactic bacteria (MTB) from complex communities, as suggested by amplified ribosomal DNA restriction analysis and denaturing gradient gel electrophoresis combined with sequence analysis of 16S rRNA genes. Using these purification methods, the occurrence and diversity of MTB ...
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In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fosmid libraries were constructed and screened by end sequencing and hybridizatio...
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Magnetotactic bacteria are characterized by the magnetosome, a membrane-bound, nano-sized organelle containing a magnetic crystal. This organelle is believed to help the bacteria to navigate along the geomagnetic field lines and find a preferred habitat for survival. The magnetosome is a biomineral structure highly controlled by the bacteria, composed of either magnetite (Fe3O4) or greigite (Fe...
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Magnetotactic bacteria (MTB) represent a diverse collection of motile prokaryotes that biomineralize intracellular, membrane-bounded, tens-of-nanometer-sized crystals of a magnetic mineral called magnetosomes. Magnetosome minerals consist of either magnetite (Fe3O4) or greigite (Fe3S4) and cause cells to align along the Earth's geomagnetic field lines as they swim, a trait called magnetotaxis. ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2005
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.71.5.2723-2731.2005